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animal facilities

 

The University of Navarra has a service of animal facilities for researchers. The animal facilities has staff veterinary and staff technical centre specialised in Animal Sciences at laboratory and it is a centre Username for experimental animals that includes the facilities located in the CIFA building, the Experimentation building and the CIMA building.

Meet the Animal Experimentation Service

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Research Management Service

 

The Research Management Service has as goal to help researchers with the different needs that may arise as a result of their activity. Its work includes support on issues ranging from the application of a subsidy, how to manage a project of research, how to organise a congress or how to achieve a scholarship for the research, among others. Its services also include the bibliometrics, Protection and Outcome Promotion Service and the translation service.

Get to know the service

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Proteomics, Genomics and Bioinformatics

01

Advice on boarding

of proteomics research

02

Realisation of gels

one- and two-dimensional staining for Coomasie, Sypro Ruby or Western Blot staining

03

Comparative analysis

of proteomes based on two-dimensional electrophoresis with DIGE technology

04

Sample processing

protein: precipitation and digestion

05

Protein identification


06

Mixing study

of medium and highly complex proteins

07

Identification of some

post-translational modifications (ESI/MS/MS) such as phosphorylations, methylations, or acetylations

08

Substance characterisation

(peptides, sugars, bile acids, etc.) using mass spectrometry

09

Characterisation of the mass

protein molecular

10

Quantitative analysis

of complex proteomes by iTRAQ tagging

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Protein quantification

through MRM

12

Quantification of metabolites

by MRM: adenosine nucleotides (APT, ADP, AMP) and polyamines (spermidine, spermine and putrescine).

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In addition, practical courses are regularly organised at training recognised by the high school Nacional de Proteómica (ProteoRed), the Spanish Proteomics Society (SeProt) and the Spanish Society of Biochemistry (SEBBM).

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Instrumental techniques: cytometry, nucleic acid extraction, sequencing

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Researchers at CIMA and the University of Navarra may use the services of the DNA Sequencing Unit, subject to the following rules:

Samples shall be sent duly identified, together with a purchase voucher and a form request form duly completed in all sections. The purchase voucher will already be completed by the researcher, indicating the issue of reactions, the issue of account to which the position will be made and the name of the researcher manager of that account. The request form is attached to this document.

To check that the amount of DNA is sufficient, it is essential to also send an image of an agarose gel of the purification products sent, indicating the loading volume of each sample. As a guideline, when 2 µL of a sample produces a visible band on a standard agarose gel, the minimum concentration of DNA in that sample can be considered to be around 5-10 ng/µL.

If sample is left over after electrophoretic separation and is not claimed by the sending researcher , it will be destroyed one week after receipt.

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Within 72 hours of receipt of the samples, the researcher will receive by e-mail the files with the electrophorograms (.ab1), which can be analysed with any .abi file viewer. No paper sequences will be sent nor will we intervene in the subsequent interpretation and/or programs of study of the obtained data .

At summary, the researcher will send to the Sequencing Unit:

  1. Samples, well labelled and with the minimum amount of DNA indicated on form.
     
  2. The duly completed form .
     
  3. An image of an agarose gel representative of the concentration of DNA in the purification products.
     
  4. A duly completed shopping voucher.

Important: It is compulsory to indicate the concentration of the sample

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