Support services
Proteomics, Genomics and Bioinformatics
01
Advice on boarding
of proteomics research
02
Realisation of gels
one- and two-dimensional staining for Coomasie, Sypro Ruby or Western Blot staining
03
Comparative analysis
of proteomes based on two-dimensional electrophoresis with DIGE technology
04
Sample processing
protein: precipitation and digestion
05
Protein identification
06
Mixing study
of medium and highly complex proteins
07
Identification of some
post-translational modifications (ESI/MS/MS) such as phosphorylations, methylations, or acetylations
08
Substance characterisation
(peptides, sugars, bile acids, etc.) using mass spectrometry
09
Characterisation of the mass
protein molecular
10
Quantitative analysis
of complex proteomes by iTRAQ tagging
11
Protein quantification
through MRM
12
Quantification of metabolites
by MRM: adenosine nucleotides (APT, ADP, AMP) and polyamines (spermidine, spermine and putrescine).
In addition, practical courses are regularly organised at training recognised by the high school Nacional de Proteómica (ProteoRed), the Spanish Proteomics Society (SeProt) and the Spanish Society of Biochemistry (SEBBM).
Instrumental techniques: cytometry, nucleic acid extraction, sequencing
Researchers at CIMA and the University of Navarra may use the services of the DNA Sequencing Unit, subject to the following rules:
Samples shall be sent duly identified, together with a purchase voucher and a form request form duly completed in all sections. The purchase voucher will already be completed by the researcher, indicating the issue of reactions, the issue of account to which the position will be made and the name of the researcher manager of that account. The request form is attached to this document.
To check that the amount of DNA is sufficient, it is essential to also send an image of an agarose gel of the purification products sent, indicating the loading volume of each sample. As a guideline, when 2 µL of a sample produces a visible band on a standard agarose gel, the minimum concentration of DNA in that sample can be considered to be around 5-10 ng/µL.
If sample is left over after electrophoretic separation and is not claimed by the sending researcher , it will be destroyed one week after receipt.
Within 72 hours of receipt of the samples, the researcher will receive by e-mail the files with the electrophorograms (.ab1), which can be analysed with any .abi file viewer. No paper sequences will be sent nor will we intervene in the subsequent interpretation and/or programs of study of the obtained data .
At summary, the researcher will send to the Sequencing Unit:
- Samples, well labelled and with the minimum amount of DNA indicated on form.
- The duly completed form .
- An image of an agarose gel representative of the concentration of DNA in the purification products.
- A duly completed shopping voucher.
Important: It is compulsory to indicate the concentration of the sample