HPLC Focused Course
June 2024 - Pamplona
This degree scroll aims to complement the training in the HPLC technique (High Performance Liquid Chromatography) very useful in the Chemistry, pharmaceutical and food industry. Through this focused course it is intended to provide the knowledge and the necessary tools for students to implement this technique in the laboratory. This course will be taught by experienced professionals who have already successfully addressed this challenge.
The course is aimed at both staff laboratory technician as well as to those professionals whose work requires them to be familiar with the exhaustive knowledge of the HPLC technique. Additionally, it is contemplated the participation of doctoral students, researchers and staff in training that may require this knowledge.
The content of the course includes the fundamental theoretical instructions in high-performance liquid chromatography and, in addition, a series of practical sessions of laboratory to learn about equipment handling, sample preparation, development of the method and troubleshooting.
Graduates of area of Sciences
training Professional of area health and industry
staff researcher at training
Technicians of laboratory
Doctoral students with technical training needs
A high percentage of science graduates work in pharma-biotech companies. The goal of this course is to train them in knowledge and skills that will facilitate their future employment rate.
In addition, this program is extended to all students of Master's Degree and doctorate in the field of experimental and health sciences (Pharmacy and Nutrition, Nursing, Medicine) and Engineering.
I) INTRODUCTION
The chromatographic process.
Types of chromatography and classification.
II) BASIC CONCEPTS
Migration and retention.
Peak parameters.
Effectiveness and resolution.
Chromatograms.
III) HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
Theory of liquid chromatography.
Description of the liquid chromatograph.
Types: Normal and reversed phase chromatography, ion chromatography. UPLC.
Mobile phase: solvent selection, miscibility, pH, mobile phase change. Isocratic and gradient elution.
Columns: classification, types of columns and selection of the most appropriate one. Use, conservation and recovery of columns. Precolumns. Characteristics of stationary phases.
Injectors: manual and automatic.
Detectors: characteristics, detection systems and selection of the most suitable detector.
Qualitative and quantitative analysis (employment of calibrated lines, separation of overlapping peaks).
IV) DEVELOPMENT OF CHROMATOGRAPHIC METHODS depending on the products to be analyzed.
V) SAMPLE PREPARATION AND PROCESSING
Liquid-liquid extraction.
Solid phase extraction.
employment of cartridges.
Filtration.
Other techniques.
VI) VALIDATION OF CHROMATOGRAPHIC METHODS
Application to the quantification of an active principle in a hypothetical stability study of an analyte in solution.
Experimental study of the parameters: university entrance exam, linearity, range, limits of detection and quantification, precision, accuracy and robustness.
Applications in the pharmaceutical industry: types of analysis that are usually performed with HPLC: assay, dissolution, related substances, etc...
VII) PRACTICAL PROGRAM
work with liquid chromatography equipment with different detectors and automatic injection. Focus most of the internship on HPLC/DAD (diode array detector).
Sample preparation.
development of a chromatographic method.
How to solve small problems (change of frits, leaks...) cleaning of equipment, etc.
Other techniques.
schedule
School of Sciences - University of Navarra (Pamplona)
Friday, June 14th:
09:00 to 14:00 h. and 16:00 to 20:00 h.
Saturday, June 15:
09:00 to 14:00 h.
Price: 895 euros
admissions period: Open until June 10
Places: 40
More information
Xabier Domínguez
xdominguez@alumni.unav.es